|Year : 2017 | Volume
| Issue : 3 | Page : 173-179
Variables associated with persistence of C-Peptide secretion among patients with Type 1 diabetes mellitus
Ibrahim Abbood Zaboon1, Abbas Ali Mansour2, Nazar S Haddad3
1 Al-Faiha Specialized Diabetes, Endocrine, and Metabolism Center, Basrah, Iraq
2 Department of Medicine, Basrah College of Medicine, Basrah, Iraq
3 Department of Biochemistry, Basrah College of Medicine, Basrah, Iraq
|Date of Web Publication||13-Jul-2017|
Abbas Ali Mansour
Al-Faiha Specialized Diabetes, Endocrine and Metabolism Center, Department of Medicine, Division of Endocrine and Metabolism, Basrah College of Medicine, P. O. Box: 142, Basrah 61013
Source of Support: None, Conflict of Interest: None
Background: C-peptide is a reliable method for estimating the beta-cell residual function. The objective of this study to assess the variables associated with persistence of C-peptide secretion among patients with Type 1 diabetes mellitus (T1DM). Patients and Methods: This was a cross-sectional study conducted from October 2015 to September 2016. This study enrolled patients with T1DM with at least 1 year or more duration. Random C-peptide with concomitant plasma glucose at least 144 mg/dl (8 mmol/l) was measured and at this cutoff considered as a stimulated value. Variables that were assessed were age at the time of enrollment, age at the diagnosis of diabetes, gender, family history of diabetes, duration of diabetes, frequency of insulin per day, insulin dose (units/kg/day), type of insulin, devices delivery, body mass index (BMI) at enrollment, blood pressure, glucose (plasma), lipid profile, glycated hemoglobin (HbA1c), thyrotropin (TSH), and antibodies to glutamic acid decarboxylase (GAD65), thyroid peroxidase antibodies (anti-TPO), and tissue transglutaminase antibodies-IgA (anti-TTG-IgA). Results: A total 324 patients were included in the study. A higher level of C-peptide has been seen if the disease acquired at the age of 18 years and older with detectable C-peptide observed among 17.7% of those diagnosed at age <18 years versus 31.7% for those aged 18 years or above. The more the duration of diabetes, the more is the loss of C-peptide. On logistic regression analysis, only duration of diabetes <6 years, and insulin dose <1 U/kg/day were statistically significantly associated with the detectable level of C-peptide in this cohort of T1DM. Conclusion: Diagnosis of TIDM at a late age, positive family history of diabetes, those requiring <1 U of insulin per kg per day, and higher fasting glucose was associated with higher and more detectable C-peptide. On multivariable analysis, the only duration of diabetes <6 years and insulin dose <1 U of insulin per kg per day remains significantly associated with detectable C-peptide after at least 1 year from the diagnosis of T1DM. The gender, the BMI, blood pressure, the number of insulin injections per day, GAD65, anti-TTG-IgA, anti-TPO antibodies together with random glucose, lipid profile, HbA1c, or TSH levels failed to predict detectable C-peptide at 1 year from the diagnosis of T1DM.
Keywords: C-peptide, persistence, Type 1 diabetes mellitus
|How to cite this article:|
Zaboon IA, Mansour AA, Haddad NS. Variables associated with persistence of C-Peptide secretion among patients with Type 1 diabetes mellitus. CHRISMED J Health Res 2017;4:173-9
|How to cite this URL:|
Zaboon IA, Mansour AA, Haddad NS. Variables associated with persistence of C-Peptide secretion among patients with Type 1 diabetes mellitus. CHRISMED J Health Res [serial online] 2017 [cited 2017 Dec 17];4:173-9. Available from: http://www.cjhr.org/text.asp?2017/4/3/173/210484
| Introduction|| |
Serum C-peptide levels significantly decreased 5 years after the diagnosis of Type 1 diabetes (T1DM). Residual C-peptide secretion is found in almost one out ofthree individuals three or more years from T1DM diagnosis among cohort in the USA, which is good sign, but indicate that this disease is heterogeneous.,
The overall frequency of detectable random C-peptide was 29%, decreasing with time from diagnosis regardless of age at diagnosis. The level of C-peptide was higher with diagnosis age >18 years compared with 18 years or less.
The C-peptide level is a surrogate of pancreatic beta-cell mass and insulin secretion and has been, thus, used for diabetes classification. About 93% of individuals have detectable C-peptide 2 years from diagnosis. In 11% of individuals, there was no significant fall from baseline by 2 years.
The objective of this study is to assess the variables associated with persistence of C-peptide secretion among patients with T1DM.
| Patients and Methods|| |
This was a cross-sectional study conducted from October 2015 to September 2016. Participants were patients with T1DM.
From all, history was taken with clinical examination to assess for the presence of exclusion criteria. Information regarding duration of disease, family history of TIDM, T2DM in the first-degree relatives, insulin frequency per day and type of insulin with device delivery (syringes, pen, human, or analog insulin) was collected.
Anthropometric parameters including weight and height were also measured with bare feet and light clothes using Seca scales, and height measuring equipment and body mass index (BMI) calculated as kg/m 2.
Blood pressure was measured using sphygmomanometer for the right arm in the sitting posture after 5 min rest.
Diabetic foot history was considered positive if the patient currently or in the past has a history of foot ulceration that necessitated local care with or without systemic treatment.
Peripheral neuropathy was diagnosed based on history with objective physical signs.
Patients with T1DM if they agree to participate and has diabetes for at least 1 year or more to avoid partial remission (honeymoon phase).
Eligible patients were clinically classified as having T1DM according to the recommendations of others, but no age excluded if he needs insulin within 1 year of diagnosis.,,
By medical history, physical examination, and routine laboratory test the following were excluded from the study:
- Renal failure (serum creatinine more than 1.4 mg/dl) because C-peptide levels must be interpreted with caution in renal failure (causes the false high level)
- Recent diabetic ketoacidosis within 1 week
- Pregnant women
- Those with random plasma glucose ≤140 mg/dL (They were asked to come next day 2 h after a meal).
Main outcome measure
Study variables associated with detectable levels of C-peptide among patients with TIDM.
Of the 364 patients, 324 were included in the study, all subjects provide verbal informed consent, and the research protocol was approved by the University of Basrah.
From each patient, 10 ml of blood was taken during the work time 8:30 am to 2 pm daily. All samples were collected in tubes containing clot activator and ethylenediaminetetraacetic acid (EDTA).
The serum was separated and stored frozen at −20 until analysis, but the plasma test for glucose and whole blood for glycated hemoglobin (HbA1c) was done on the same day of collection.
Grossly hemolytic, lipemic, or icteric samples were excluded from the study.
Fully automated chemiluminescence immunoassay kits, Cobas e411 analyzer series, Roche Diagnostics, Germany, were used for the assay of C-peptide. The performance characteristics of the assay were measuring range 0.01–40 ng/ml (0.003–13.3 nmol/l) with specified intraassay precision 0.5–20 ng/ml, and total variation (sum of intra- and inter-assay variation) was 7%. The normal values kits C-peptide = 0.9–4.3 ng/ml ([0.29–1.43 nmol/L] which mean that 1 ng/mL = 0.333 nmol/L and ng/mL × 331 = C-peptide concentration in pmol/L).
As C-peptide was measured randomly regarding meal consumption at the diagnosis of T1DM, it was considered stimulated.
The preservation of C-peptide, indicating residual beta-cell function, was defined as a stimulated C-peptide level ≥0.6 ng/mL (0.2 nmol/L).
C-peptide and glucose were measured within 7 h of blood sampling to avoid loss of stability. Concomitant serum glucose should be >144 mg/dl (8 mmol/L) because glucose less than that level will suppress insulin and C-peptide) and at this cutoff considered as a stimulated value.
Glucose and lipid profile were measured by clinical chemistry analyzer Bialyzer 300. Lipid estimation was done after at least 8–12 h fast in the morning. Random plasma glucose was measured at the same time of C-peptide, and definition of random was plasma glucose measured at least 2 h from the beginning of the last meal up to <8 h.
Antibodies against the glutamic acid decarboxylase (GAD65) were measured by fully automated chemiluminescent immunoassay MAGLUMI 2000 kit. The measuring range was 1.0–280.0 IU/mL. The cutoff for a positive test is ≥30 IU/mL, and specified the intraassay precision of 12.91 ± 2.75 IU/mL (4.36% CV).
HbA1c was measured by BioRad–D10, high performance liquid chromatography system using blood collected in EDTA tube on the same day of a collected of blood for above biochemical tests.
Thyrotropin (TSH) and thyroid peroxidase antibodies (anti-TPO) were measured in all patients to assess the presence of thyroid autoimmunity using chemiluminescence immunoassay kits Cobas e411 analyzer series (Roche Diagnostics, Germany). Elecsys TSH test is a 3rd generation TSH test. TSH ranged 0.005–100.0 μIU/mL with a reference range of 0.27–4.2 μIU/ml and specified the intraassay precision of 0.1–4 μU/mL (<5% CV). Anti-TPO measuring range 5–600 IU/ml with positive values >34 IU/ml and specified the intraassay precision of >40 IU/ml (<7% CV).
Tissue transglutaminase antibodies-IgA (Anti-TTG-IgA) to screen for associated celiac disease was measured using ELISA (AESKULISA ® TTG A). The analytical sensitivity (detection limit) was 1.0 U/Ml. The cutoff value was 15 U/mL for the anti-TTG A. The intraassay precision was 13.8–166.5 U/mL (5.2%–7.0% CV).
All data were computed and analyzed using SPSS, (version 23.0, SPSS Inc., Chicago, IL, USA). Continuous variables were summarized as the mean (standard deviation), and categorical variables were summed up as a percentage.
Logistic binary regression was used to see the independent variables significantly associated with detectable C-peptide on univariate analysis.
Statistical significance indicated by a value of P < 0.05.
| Results|| |
Of the 364 patients, 324 were included in the study and 40 patients were excluded because not completed their investigations.
[Table 1] shows baseline characteristics of 324 patients enrolled in this study (180 [55.4%] of them male and 145 [44.6%] female). The age at the diagnosis of T1DM ranges 1–46 year (mean 16.6 ± 8.2 year). Of them, 198 (61.1) patients were below the age of 18 years while 126 (38.9) patients were 18-year-old or more with age at enrollment in the study range from 3 to 50 year. The male constitutes 180 (55.4%) patients, and 145 (44.6%) patients were female. BMI ranges 13.3–38.6 kg/m 2 (mean 23.0 ± 4.6). The family history of diabetes was positive in 105 patients (29.5%) (Whether TIDM of T2DM). The total duration of diabetes was range from 1 to 36 years (mean 7.4 ± 5.9). All patients used insulin therapy with range 2–5 injections per a day (mean 3.5 ± 0.6). Total insulin dose per day ranged 12–160 unit (mean 62.0 ± 22.7). Most of them used human insulin using syringes. The diabetic foot was observed in 18 (5.6) patients, and peripheral neuropathy based on clinical examination was seen in 116 (35.8%) patients.
[Table 2] shows baseline biochemical tests. The mean random and fasting serum glucose was 287.8 ± 101.9 mg/dL and 231.5 ± 109.4 mg/dL, respectively, and the mean C-peptide was 0.49 ± 1.03 ng/mL (0.16–0.34 nmol/L). The mean of total cholesterol, triglyceride, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol, and very LDL-C were 173.9 ± 46.1 mg/dL, 133.9 ± 106.7 mg/dL, 101.7 ± 31.5, 101.7 ± 31.5 mg/dL, and 46.9 ± 13.8 mg/dL, respectively. The mean HbA1c was 10.5% ±2.7%. There were 101 patients (31.2%) GAD65 antibody positive, anti-TPO positive in 58 (17.9%), and 37 patients (11.4%) were positive for anti-TTG-IgA. The mean TSH was 3.7 ± 9.1 μU/mL, and mean C-peptide was 0.49 ± 1.03 ng/mL (0.16–0.34 nmol/L).
[Table 3] shows the mean level of C-peptide at the age of disease onset <18 years and those more. A higher level of C-peptide was seen if the disease acquired at age 18 years or more.
|Table 3: C-peptide levels- according to the age ranges below 18 years and 18 years or more|
Click here to view
[Table 4] shows C-peptide levels according to the age ranges (below 18 years and 18 years or more). About 23.1% of the whole cohort has detectable C-peptide. Detectable C-peptide which mean ≥0.6 ng/mL (0.2 nmol/L) was seen in 17.7% of those diagnosed at age <18 years versus 31.7% for those aged 18 years or more that with statistically significant results (P = 0.003). The detectable C-peptide level was more in those with diabetes duration <6 years (33.6.1% vs. 14.9%). This was the strongly significant difference (P< 0.0001). Those with positive family history were more likely to have detectable C-peptide that was statistically significant (P = 0.001). Those with a total daily dose of insulin <1 U/kg/day were more likely to have detectable C-peptide (34.9% vs. 13.5%) with a P< 0.0001. GAD65 antibody positivity dose not associated with the loss of C-peptide. There was no difference in positivity to anti-TTG-IgA in the level of C-peptide. The level of anti-TPO had no effect on the C-peptide level.
[Figure 1] shows C-peptide levels according to 6 strata of the duration of diabetes in years. It seems that the more the duration, the more the loss of C-peptide, which was statistically significant (P = 0.002).
|Figure 1: C-peptide levels according to 6 strata of duration of diabetes in years (P = 0.002)|
Click here to view
[Table 5] shows C-peptide levels according to contentious variables.
Only fasting serum glucose and BMI were higher in those with higher C-peptide. None of the other studied variables were statistically significantly associated with higher C-peptide including blood pressure, random glucose, lipid levels, HbA1c, or TSH.
[Table 6] shows logistic binary regression for variables significantly associated with detectable C-peptide on univariate analysis. Only duration of diabetes <6 years, and insulin dose <1 U/kg/day were statistically significantly associated with the detectable level of C-peptide in this cohort of T1DM.
|Table 6: Logistic binary regression for variables significantly associated with detectable C-peptide on univariate analysis|
Click here to view
| Discussion|| |
About 61.1% of patients enrolled in this study had disease onset below the age of 18 years which confirm adolescence prevalence of the disease like that seen by others.,
Family history of diabetes was positive 29.5% of our patients (both for T1DM or T2DM) while in Hemminki et al. study, it was positive in approximately 10% of patients with T1DM.
The total daily insulin dose in the study was 1.06 ± 0.34 with very wide range (0.30–2.5). This is slightly higher than a recommendation, probably because diet education is not integral part of care of diabetes in the third world and the reliance mainly on insulin for glycemic control.,
Human insulin was the only type of insulin used in most patients in this study. This seems to be a global problem worldwide because of the cost of insulin analogs.
Peripheral neuropathy on clinical criteria was seen in 35.8% and diabetic foot history in 5.6%. Neuropathy was seen in 8.2% among youth with T1DM in the USA cohort.,
The mean HbA1c in this study was 10.5% ±2.7%. This reflects poor glycemia which was also seen before.,
About 31.2% of patients in this study were positive for the GAD65 antibody. The prevalence GAD65 antibody was very variable ranges 63%–80%., From the same center, of diabetics with age 30 years and more, 26.4% were glutamic acid decarboxylase autoantibodies-positive.
About 17.9% of those tested were anti-TPO positive, which was comparable to other studies, where the prevalence of antithyroid antibodies in children with T1DM at disease onset is about 18%–20%.
About 11.4% of the patients tested for anti-TTG-IgA were positive. The prevalence of anti-TTG-IgA in patients with T1DM is variable. In Egypt, it was seen among 5.4% to 7% in the Czech Republic  and reaching to 14.5% in another Iraqi study.
The mean C-peptide was statistically higher among those diagnosed with diabetes at the age of 18 and more. This conclusion was compatible with the newest US study, where diagnosis during adulthood is associated with greater frequency and higher values of C-peptide. We found that 23.1% of our cohort has detectable C-peptide after at least 1 year after diagnosis of T1DM. However, in US cohort, one out of three has detectable C-peptide.
As regards C-peptide levels, 17.7% of those diagnosed with diabetes before the age of 18 years versus 31.7% of those diagnosed at the age of 18 years or more had detectable C-peptide. This mean diagnosing T1DM at age 18 years or more were associated with more detectable C-peptide and late diagnoses at age 18 years and above associated with less loss of beta cells, which goes with that of study in Belgium and Sweden where younger patients at diagnosis lost more C-peptide after a while., Earlier age at diagnosis associated with more loss of a C-peptide level in Spain study. and age at diagnosis is regarded as important cofounder for the persistence of detectable C-peptide. The results of this study were consistent with the USA study diagnosis in adulthood was associated with better and higher C-peptide level.
The more the duration of diabetes, the less the C-peptide level in this study. This relation seems linear over 10 year-period and more. and was similar to other studies.
Duration of diabetes more than 6 years were strongly associated with loss of C-peptide. This was considered as strong predictor of loss beta cells function and subsequent treatment to preserve beta cells and prevention complications in T1DM.,,
As regards the family history of diabetes, detectable C-peptide in this study was more in those with a family history of diabetes. While in Korean study, family history of diabetes was significantly associated with the progressive decline of fasting plasma C-peptide levels, but that was in patients with T2DM.
It seems to be common sense to have more detectable C-peptide in those who need <1 U/kg/day of insulin, and this was the same as we have seen in this study.
None of the autoantibodies screened in our patients predict the persistence of C-peptide after 1 year of T1DM. This was applied for the GAD65 antibody, anti-TTG-IgA, and anti-TPO. The occurrence of autoantibodies did not correlate with C-peptide decline, except possibly for a more rapid loss in insulin autoantibody-positive patients in Sweden study.
The fasting glucose was higher in those with detectable C-peptide may be explained on the basis of mild insulin resistance, but this was not seen before.
| Conclusion|| |
Diagnosis of TIDM at a late age, positive family history of diabetes, those requiring <1 U of insulin per kg per day, higher BMI, and higher fasting glucose was associated with higher and more detectable C-peptide. On multivariable analysis, the only duration of diabetes <6 years and insulin dose <1 U of insulin per kg per day remains significantly associated with detectable C-peptide after at least 1 year from the diagnosis of T1DM. The gender, the BMI, blood pressure, the number of insulin injections per day, GAD65, anti-TTG-IgA, anti-TPO antibodies together with random glucose, lipid profile, HbA1c, or TSH levels failed to predict detectable C-peptide at 1 year from the diagnosis of T1DM.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
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[Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6]