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 Table of Contents  
CASE REPORT
Year : 2015  |  Volume : 2  |  Issue : 4  |  Page : 367-369

Leuconostoc lactis: An unusual cause for bacteremia


Department of Microbiology, Institute of Medical Sciences and SUM Hospital, SOA University, Bhubaneswar, Odisha, India

Date of Web Publication18-Sep-2015

Correspondence Address:
Bichitrananda Swain
HIG 5/4 Ext. Phase-1, Housing Board Colony, Chandrasekharpur, Bhubaneswar - 751 016, Odisha
India
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Source of Support: Nil., Conflict of Interest: There are no conflicts of interest.


DOI: 10.4103/2348-3334.165747

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  Abstract 

Leuconostoc species, the common saprophytic bacteria are now emerging as one of the important opportunistic pathogens. There are different risk factors such as compromised immunity, use of vancomycin, parenteral nutrition, and contaminated surgical implanted devices to contribute the human infection. We here present one of the unusual fatal cases of bacteremia in a cancer patient who was infected with a vancomycin-resistant strain of L. lactis.

Keywords: Bacteremia, blood culture, Leuconostoc lactis


How to cite this article:
Swain B, Sahu KK, Rout S. Leuconostoc lactis: An unusual cause for bacteremia. CHRISMED J Health Res 2015;2:367-9

How to cite this URL:
Swain B, Sahu KK, Rout S. Leuconostoc lactis: An unusual cause for bacteremia. CHRISMED J Health Res [serial online] 2015 [cited 2019 Sep 23];2:367-9. Available from: http://www.cjhr.org/text.asp?2015/2/4/367/165747


  Introduction Top


Leuconostoc species, belonging to the genus II of the family Streptococcaceae [1] are usually found on herbage, vegetables, dairy products, and wine and sugar solutions. Leuconostoc lactis, one of the members in this family with a previous impression was nonpathogenic to humans may occasionally cause ventriculitis,[2] osteomyelitis,[3] and blood stream infection.[4],[5],[6] More recently these Gram-positive cocci have been recognized as the potential pathogens causing opportunistic infections.

With the increased use of vancomycin in clinical practice, some new vancomycin-resistant pathogenic bacteria are emerging. Although Leuconostoc infections have also been documented in otherwise healthy patients; compromised immunity,[7],[8] use of vancomycin, parenteral nutrition and contaminated surgical implanted devices, such as central venous catheters have been considered as risk factors for this type of infection.[9] Prompt and accurate identification of Leuconostoc species and knowledge regarding clinical significance of these potential pathogens is essential during treating the patient for a better outcome.


  Case Report Top


A 62-year-old male from Western Odisha, a known endemic area for Plasmodium falciparum infection admitted in our hospital with high-grade fever for 10 days had a history of Type 2 diabetes mellitus and hypertension since 4 years. He was also under treatment for myelofibrosis, a rare bone marrow cancer. He had pancytopenia with low hemoglobin level (5 g%) and biochemical parameters such as serum creatinine (2 mg/dL), urea (56 mg/dL), and sodium (160 mg/dL) were in the higher range. His tests for malaria parasite were negative. He had a very low blood pressure (90/60 mmHg) with feeble pulse rate. After excluding malaria parasite infection, empiric parenteral administration of vancomycin along with correction of electrolytes and blood transfusion was initiated. Subsequently, clean-catched midstream urine and two sets of blood samples in consecutive days were sent to our department for aerobic bacterial culture and sensitivity. Urine culture was negative by conventional method whereas repeated blood cultures were positive by automated Bact/ALERT 3D (Biomerieux) method. By doing subculture from the later, pure nonhaemolytic tiny bacterial colonies were isolated on blood agar [Figure 1] without showing any growth on MacConkey agar. The isolates from both the blood cultures were initially included in the Streptococcus viridians group as they were Gram-positive cocci and coccobacillary forms and arranged in pairs and chains. They were catalase and bile esculin negative. Antimicrobial susceptibility test was performed according to Clinical and Laboratory Standards Institute guideline on blood agar [Figure 2] by Kirby–Bauer disk diffusion method. Further, identification of the isolate was done by Vitek-2 Compact automated system (BioMerieux, France) using GP card. The organism was confirmed as L. lactis. In our case, the patient died before changing to suitable antibiotics, who was empirically treated with vancomycin for 48 h to which the bacterium showed resistance.
Figure 1: Nonhemolytic colonies of Leuconostoc lactis on blood agar

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Figure 2: Antimicrobial susceptibility test on sheep blood agar

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  Discussion Top


Bacteremia due to Leuconostoc spp. remains an uncommon clinical entity. Owing to the paucity of documented cases, the exact mode of transmission to humans and the pathogenesis of Leuconostoc infections remain poorly defined. Further, identification and reporting of Leuconostoc spp. is lacking. This is partly, because of the infrequent occurrence and sharing of many morphological and biochemical characteristics with viridians streptococci. Management of this infection becomes really difficult because of diagnostic difficulties and its intrinsic resistance to vancomycin.

Possible predisposing factors for Leuconostoc bacteremia include immunosuppression, prolonged hospitalization, presence of a central venous catheter, need for total parenteral nutrition, disruption of bowel mucosa, and long-term antibiotic treatment especially with vancomycin. In our case, as the patient had myelofibrosis, which might have increased susceptibility to different types of infection. Further, the outcome was worsened due to the bacterial intrinsic resistance to the vancomycin with which he was empirically treated.

Bacteremia due to Leuconostoc spp. remains an uncommon clinical entity. In our case, the patient died before changing to other antibiotics from the vancomycin as it was resistant to the later. Successful treatment strategies include removal of indwelling catheters and administration of intravenous penicillin [10] Alternative antimicrobials that have been suggested include clindamycin, ampicillin, macrolides, minocycline, aminoglycosides, and more recently daptomycin and tigecycline.[11],[12] When culture yields nonenterococcal Gram-positive cocci in pairs and chains with a decreased susceptibility to vancomycin, infection due to Leuconostoc spp. should always be suspected. Since, vancomycin remains a commonly used choice of empiric therapy for bacteremia with Gram-positive organisms, efforts should be made to alert clinicians of the possibility of vancomycin-resistant Leuconostoc spp. as soon as possible to prevent any delay in initiation of appropriate antibiotic therapy in patients.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

 
  References Top

1.
Garvie EI, Leuconostoc. In: Buchanan RE, Gibbons NE, editors. Bergey's Manual of Determinative Bacteriology. 8th ed. Baltimore: The Williams and Wilkins Co.; 1974. p. 510-3.  Back to cited text no. 1
    
2.
Deye G, Lewis J, Patterson J, Jorgensen J. A case of Leuconostoc ventriculitis with resistance to carbapenem antibiotics. Clin Infect Dis 2003;37:869-70.  Back to cited text no. 2
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3.
Koçak F, Yurtseven N, Aydemir N, Yüksek A, Yavuz SS. A case of osteomyelitis due to Leuconostoc lactis. Scand J Infect Dis 2007;39:278-80.  Back to cited text no. 3
    
4.
Shin J, Her M, Moon C, Kim D, Lee S, Jung S. Leuconostoc bacteremia in a patient with amyloidosis secondary to rheumatoid arthritis and tuberculosis arthritis. Mod Rheumatol 2011;21:691-5.  Back to cited text no. 4
    
5.
Deng Y, Zhang Z, Xie Y, Xiao Y, Kang M, Fan H. A mixed infection of Leuconostoc lactis and vancomycin-resistant Enterococcus in a liver transplant recipient. J Med Microbiol 2012;61:1621-4.  Back to cited text no. 5
    
6.
Vagiakou-Voudris E, Mylona-Petropoulou D, Kalogeropoulou E, Chantzis A, Chini S, Tsiodra P, et al. Multiple liver abscesses associated with bacteremia due to Leuconostoc lactis. Scand J Infect Dis 2002;34:766-7.  Back to cited text no. 6
    
7.
Espinoza R, Kusne S, Pasculle AW, Wada S, Fung J, Rakela J. Leuconostoc bacteremia after liver transplantation: Another cause of vancomycin resistant Gram-positive infection. Clin Transplant 1997;11:322-4.  Back to cited text no. 7
    
8.
Ferrer S, de Miguel G, Domingo P, Pericas R, Prats G. Pulmonary infection due to Leuconostoc species in a patient with AIDS. Clin Infect Dis 1995;21:225-6.  Back to cited text no. 8
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9.
Bou G, Luis Saleta J, Sáez Nieto JA, Tomás M, Valdezate S, Sousa D, et al. Nosocomial Outbreaks Caused by Leuconostoc mesenteroides subsp. mesenteroides. Emerg Infect Dis 2008;14:968-71.  Back to cited text no. 9
    
10.
Handwerger S, Horowitz H, Coburn K, Kolokathis A, Wormser GP. Infection due to Leuconostoc species: Six cases and review. Rev Infect Dis 1990;12:602-10.  Back to cited text no. 10
    
11.
Golan Y, Poutsiaka DD, Tozzi S, Hadley S, Snydman DR. Daptomycin for line-related Leuconostoc bacteraemia. J Antimicrob Chemother 2001;47:364-5.  Back to cited text no. 11
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12.
Lee MR, Huang YT, Lee PI, Liao CH, Lai CC, Lee LN, et al. Healthcare-associated bacteraemia caused by Leuconostoc species at a university hospital in Taiwan between 1995 and 2008. J Hosp Infect 2011;78:45-9.  Back to cited text no. 12
    


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